7-TD-1

Cat.No.: CSC-C1056

Species: Mouse

Source: B cell hybridoma

Morphology: round cells, growing single or clustered in suspension

Culture Properties: suspension

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C1056

Description

Murine myeloma cells fused with C57Bl/6 spleen cells; indicator cell line for IL-6

Species

Mouse

Source

B cell hybridoma

Recommended Medium

15% + 5% ConA spleen cell supernatant (or 10 ng/ml IL-6) + 2.5 x 10-5 M mercaptoethanol + 2 mM L-glutamine

Culture Properties

suspension

Morphology

round cells, growing single or clustered in suspension

Karyotype

Murine karyotype

Quality Control

Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: bioassay for IL-6 (low cell densities are essential for reliable assays; try doubling cell di

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 9 x 10^5 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Ask a Question