2A1

Cat.No.: CSC-C6153X

Species: Mouse

Source: hybridoma (anti-chicken retina)

Morphology: round cells growing singly in suspension or loosely adherent

Culture Properties: suspension

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Cat.No.

CSC-C6153X

Description

Established by fusion of mouse NS-1 cells with spleen from a BALB/c ByI mouse immunized with White Leghorn chicken embryonic retinal and tectal membranes; secretes 2A1 monoclonal antibody which binds epitopes involved in chicken retinal morphogenesis

Species

Mouse

Source

hybridoma (anti-chicken retina)

Recommended Medium

80% RPMI-1640 I-1640 + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1-2 weeks post-thawing

Culture Properties

suspension

Morphology

round cells growing singly in suspension or loosely adherent

Karyotype

Murine hypertetraploid karyotype

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of a IgM monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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