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PC-3M
Cat.No.: CSC-C9578L
Species: Human
Source: prostate
Culture Properties: monolayer
- Specification
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vWA: 17
FGA: 24
Amelogenin: X
TH01: 6,7
TPOX: 8,9
CSF1P0: 11
D5S818: 13
D13S317: 11
D7S820: 8,11
Shipping Condition: Room Temperature
In a transmission electron microscope (TEM), electrons are emitted from an electron gun and accelerated using an electric field. The electron beam then passes through a series of electromagnetic lenses that focus the electrons onto the specimen. As the beam interacts with the specimen, it undergoes various interactions, leading to the formation of an image.
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Average Rating: 4.0 | 1 Scientist has reviewed this product
Smooth progress
I am very grateful to Creative Bioarray for the detailed instructions and cytology-related information, which made the experimental process straightforward.
12 Apr 2023
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Description: prostate epithelial cells from a 68-year-old man with benign prostate hyperplasia; cells were immortalized with SV-40 large T-antigen; cells were described to express cytokeratins 8, 18, and 19 (but not 14) and to metabolize prostatic androgens
Description: Cytogenetic analysis and DNA fingerprinting at the Creative Bioarray documented that this cell line is identical with the human prostate carcinoma-derived cell line DU-145, but shows some phenotypical differences; DU-145 was established from the tumor tissue removed from the metastatic central nervous system lesion of a 69-year-old man with prostate carcinoma in 1975
Description: These cells are responsive to 5-alpha-dihydrotestosterone (growth modulation and acid phosphatase); the cells do not produce a uniform monolayer, but grow in clusters which should be broken apart by repeated pipetting when subcultures are prepared; they attach only lightly to the substrate, do no become confluent and rapidly acidify the medium; growth is very slow; the cells should be allowed to incubate undisturbed for the first 48 hours after subculture; when flask cultures are shipped, the majority of the cells become detached from the flask and float in the medium. Upon receipt, incubate the flask (in the usual position for monolayer cultures) for 24 to 48 hours to allow the cells to re-attach. The medium can then be removed and replaced with fresh medium. If desired the contents of the flask can be collected, centrifuged at 300xg for 15 minutes, resuspended in 10ml of medium and dispensed into a single flask.
