LS 123

Cat.No.: CSC-C9455J

Species: Homo sapiens (human)

Specification
Q & A
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Cat.No.

CSC-C9455J

Description

Established in 1974 from the second in a series of 3 primary colonic tumours resected from a 65 year old Caucasian female, which was classified as a Duke's B mucin producing colon adenocarcinoma. LS 123 is aneuploid, fails to grow progressively in nude mice and forms colonies only in enriched semisolid medium developed for tumour stem cells. The cells will grow in either low serum or serum free medium. In chick embryonic skin assays the cells grow as a well differentiated abnormal colonic epithelium with little mitotic activity and low invasiveness. LS 123 exhibits several properties associated with neoplasia, but have little invasive potential and may represent an early stage in tumour development.

Species

Homo sapiens (human)

Tissue of Origin

Colon

Recommended Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS) (Heat inactivated)

Storage

Liquid Nitrogen (-180 °C).

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the role of vitamins in the cell culture medium?

It mainly plays the role of coenzyme and cofactor and is essential.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Fantastic

Creative Bioarray's cell products and services are fantastic.

19 Apr 2022

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For research use only. Not for any other purpose.