COLO 320 HSR

Cat.No.: CSC-C9363L

Species: Homo sapiens (human)

Morphology: rounded and refractile

Culture Properties: The cells grows loosely attached and in suspension

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Cat.No.

CSC-C9363L

Description

CEA(-), CSAp(-), colon antigen 3(+); This line is a subline of COLO 320DM. The cells are weakly positive for keratins and vimentinTumorigenecity:yes, in nude miceIsoenzyme:PGM1, 1; PGM3, 2; G6PD, B; PEP-D, 1; 6PGD, A;ES-D, 1Histopathology:adenocarcinoma; DukesSubculture:Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol. Subculture once per week.Split ratio:Media change:Reverse transcritase:negativeProductionserotonin; norepinephrine; epinephrine; adrenocorticotropic hormone(ACTH); parathyroid hormone

Species

Homo sapiens (human)

Tissue of Origin

Colon

Recommended Medium

90% RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3 + 10% h.i. FBS

Culture Properties

The cells grows loosely attached and in suspension

Morphology

rounded and refractile

STR DNA Profile

Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11/12
D5S818: 12
D7S820: 9/12
THO1: 9
TPOX: 8/9
vWA: 15/18
D3S1358: 17
D21S11: 33.2
D18S51: 15
Penta E: 11
Penta D: 9/12
D8S1179: 13
FGA: 20 , 2010.

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How do tumor cells appear?

Tumor cells are produced by normal cells due to mutation of proto-oncogenes and oncogenes caused by physical, chemical, viral and other oncogenic factors.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

High quality

I am absolutely thrilled with the quality of the cellular product I received!

14 Dec 2022

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For research use only. Not for any other purpose.