ActoFactor™ Recombinant Human Interleukin 8 (77 aa variant)

Specification

Cat.No.

CSC-CTK0239

Description

Recombinant Human Endothelial Interleukin-8 (rHu IL-8) is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells, and plays a role in chemoattraction and activation of neutrophils. Rhu IL-8 (endothelial-derived) is an 8.9 kDa protein containing 77 amino acid residues.

Species

Human

Product Overview

Human IL8 expressed in E.coli

Molecular Mass

8.9 kDa

Size

CAT# CSC-CTK0239-25 (25 μg); CAT# CSC-CTK0239-125 (125 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

Human IL-8 produces chemotaxis over a wide concentration range in an assay using human peripheral blood neutrophils. Significant chemotaxis was achieved using a concentration range of 25.0 -150.0 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized protein with no additives.

Reconstitution

Please centrifuge product briefly before opening the vial. The lyophilized powder should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into water or other buffered solutions and stored at -20°C for future use.

Storage & Stability

The lyophilized IL-8, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted human IL-8 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Please avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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