ActoFactor™ Recombinant Human Interleukin 8 (72 aa variant)

Specification

Cat.No.

CSC-CTK0240

Description

Recombinant Human Interleukin-8 (rHu IL-8) is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells, and plays a role in chemoattraction and activation of neutrophils. Rhu IL-8 (monocyte- derived) is an 8.4 kDa protein containing 72 amino acid residues.

Species

Human

Product Overview

Human IL8 expressed in E.coli

Molecular Mass

8.4 kDa

Size

CAT# CSC-CTK0240-25 (25 μg); CAT# CSC-CTK0240-125 (125 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE, RP-HPLC and AEX-FPLC.

Activity

The biological activity was determined by its ability to chemoattract human peripheral blood neutrophils. The concentration for this effect was found to be in the range of 10.0 to 100.0 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized protein with no additives.

Reconstitution

Please centrifuge product briefly before opening the vial. The lyophilized IL-8 should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted IL-8 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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