About Us
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Cell Services
- Cell Line Authentication
- Cell Surface Marker Validation Service
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Cell Line Testing and Assays
- Toxicology Assay
- Drug-Resistant Cell Models
- Cell Viability Assays
- Cell Proliferation Assays
- Cell Migration Assays
- Soft Agar Colony Formation Assay Service
- SRB Assay
- Cell Apoptosis Assays
- Cell Cycle Assays
- Cell Angiogenesis Assays
- DNA/RNA Extraction
- Custom Cell & Tissue Lysate Service
- Cellular Phosphorylation Assays
- Stability Testing
- Sterility Testing
- Endotoxin Detection and Removal
- Phagocytosis Assays
- Cell-Based Screening and Profiling Services
- 3D-Based Services
- Custom Cell Services
- Cell-based LNP Evaluation
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Stem Cell Research
- iPSC Generation
- iPSC Characterization
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iPSC Differentiation
- Neural Stem Cells Differentiation Service from iPSC
- Astrocyte Differentiation Service from iPSC
- Retinal Pigment Epithelium (RPE) Differentiation Service from iPSC
- Cardiomyocyte Differentiation Service from iPSC
- T Cell, NK Cell Differentiation Service from iPSC
- Hepatocyte Differentiation Service from iPSC
- Beta Cell Differentiation Service from iPSC
- Brain Organoid Differentiation Service from iPSC
- Cardiac Organoid Differentiation Service from iPSC
- Kidney Organoid Differentiation Service from iPSC
- GABAnergic Neuron Differentiation Service from iPSC
- Undifferentiated iPSC Detection
- iPSC Gene Editing
- iPSC Expanding Service
- MSC Services
- Stem Cell Assay Development and Screening
- Cell Immortalization
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ISH/FISH Services
- In Situ Hybridization (ISH) & RNAscope Service
- Fluorescent In Situ Hybridization
- FISH Probe Design, Synthesis and Testing Service
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FISH Applications
- Multicolor FISH (M-FISH) Analysis
- Chromosome Analysis of ES and iPS Cells
- RNA FISH in Plant Service
- Mouse Model and PDX Analysis (FISH)
- Cell Transplantation Analysis (FISH)
- In Situ Detection of CAR-T Cells & Oncolytic Viruses
- CAR-T/CAR-NK Target Assessment Service (ISH)
- ImmunoFISH Analysis (FISH+IHC)
- Splice Variant Analysis (FISH)
- Telomere Length Analysis (Q-FISH)
- Telomere Length Analysis (qPCR assay)
- FISH Analysis of Microorganisms
- Neoplasms FISH Analysis
- CARD-FISH for Environmental Microorganisms (FISH)
- FISH Quality Control Services
- QuantiGene Plex Assay
- Circulating Tumor Cell (CTC) FISH
- mtRNA Analysis (FISH)
- In Situ Detection of Chemokines/Cytokines
- In Situ Detection of Virus
- Transgene Mapping (FISH)
- Transgene Mapping (Locus Amplification & Sequencing)
- Stable Cell Line Genetic Stability Testing
- Genetic Stability Testing (Locus Amplification & Sequencing + ddPCR)
- Clonality Analysis Service (FISH)
- Karyotyping (G-banded) Service
- Animal Chromosome Analysis (G-banded) Service
- AAV Biodistribution Analysis (RNA ISH)
- Molecular Karyotyping (aCGH)
- Droplet Digital PCR (ddPCR) Service
- Digital ISH Image Quantification and Statistical Analysis
- SCE (Sister Chromatid Exchange) Analysis
- Biosample Services
- Histology Services
- Exosome Research Services
- In Vitro DMPK Services
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In Vivo DMPK Services
- Pharmacokinetic and Toxicokinetic
- PK/PD Biomarker Analysis
- Bioavailability and Bioequivalence
- Bioanalytical Package
- Metabolite Profiling and Identification
- In Vivo Toxicity Study
- Mass Balance, Excretion and Expired Air Collection
- Administration Routes and Biofluid Sampling
- Quantitative Tissue Distribution
- Target Tissue Exposure
- In Vivo Blood-Brain-Barrier Assay
- Drug Toxicity Services
Protocol for the Differentiation of Mouse Neural Stem Cells into Neurons
GUIDELINE
The generation of the different neural lines originates in adult neural stem cells that can self-renew or differentiate into astrocytes, oligodendrocytes, or neurons in response to specific stimuli. The abilities of stem cells to self-renew and form different mature cells expand the possibilities of applications in cell-based therapies such as tissue re-composition in regenerative medicine, drug screening, and treatment of neurodegenerative diseases.
METHODS
- Place a 12-mm cell culture slide in each well of a 4-well plate, add 100 μg/mL of PDL 500 μL to each well, and leave overnight at room temperature.
- The PDL is aspirated, and the slides are rinsed with sterile water and left to dry at room temperature. 10 μg/mL of laminin 500 μL is added to each well, and the slides are allowed to stand overnight at 37°C and 5% CO2.
- Mouse neural stem cells are digested with 0.05% trypsin for 2 min and terminated with an equal volume of 10% FBS. Centrifugation is performed at 1000 rpm for 5 min, the supernatant is discarded and the cells are resuspended with Neuron M. The cells are inoculated at a cell density of 1×104 cells/ml in a four-well plate in which laminin is discarded.
- Neuron M is replaced in half quantities every 2 days, and by day 21, differentiation is complete, and neuronal cells with neurofilaments can be observed microscopically.
Creative Bioarray Relevant Recommendations
- Creative Bioarray provides our customers with a large and unique collection of high-purity, low-passage human and animal neural stem cells, including but not limited to Strain C57BL/6 Mouse Neural Stem Cells, Sprague-Dawley (SD) Rat Neural Stem Cells, Human Neural Stem Cells-cortex region, Human iPSC-Derived Neural Stem Cells, Human Neural Stem Cells-ventral mesencephalon region, Mouse Spinal Cord Neural Stem Cells, Rat Hippocampal Neural Stem Cells, and others.
NOTES
Do not expose cells to air at any time after they have differentiated into neurons.
RELATED PRODUCTS & SERVICES
For research use only. Not for any other purpose.
