About Us
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Cell Services
- Cell Line Authentication
- Cell Surface Marker Validation Service
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Cell Line Testing and Assays
- Toxicology Assay
- Drug-Resistant Cell Models
- Cell Viability Assays
- Cell Proliferation Assays
- Cell Migration Assays
- Soft Agar Colony Formation Assay Service
- SRB Assay
- Cell Apoptosis Assays
- Cell Cycle Assays
- Cell Angiogenesis Assays
- DNA/RNA Extraction
- Custom Cell & Tissue Lysate Service
- Cellular Phosphorylation Assays
- Stability Testing
- Sterility Testing
- Endotoxin Detection and Removal
- Phagocytosis Assays
- Cell-Based Screening and Profiling Services
- 3D-Based Services
- Custom Cell Services
- Cell-based LNP Evaluation
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Stem Cell Research
- iPSC Generation
- iPSC Characterization
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iPSC Differentiation
- Neural Stem Cells Differentiation Service from iPSC
- Astrocyte Differentiation Service from iPSC
- Retinal Pigment Epithelium (RPE) Differentiation Service from iPSC
- Cardiomyocyte Differentiation Service from iPSC
- T Cell, NK Cell Differentiation Service from iPSC
- Hepatocyte Differentiation Service from iPSC
- Beta Cell Differentiation Service from iPSC
- Brain Organoid Differentiation Service from iPSC
- Cardiac Organoid Differentiation Service from iPSC
- Kidney Organoid Differentiation Service from iPSC
- GABAnergic Neuron Differentiation Service from iPSC
- Undifferentiated iPSC Detection
- iPSC Gene Editing
- iPSC Expanding Service
- MSC Services
- Stem Cell Assay Development and Screening
- Cell Immortalization
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ISH/FISH Services
- In Situ Hybridization (ISH) & RNAscope Service
- Fluorescent In Situ Hybridization
- FISH Probe Design, Synthesis and Testing Service
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FISH Applications
- Multicolor FISH (M-FISH) Analysis
- Chromosome Analysis of ES and iPS Cells
- RNA FISH in Plant Service
- Mouse Model and PDX Analysis (FISH)
- Cell Transplantation Analysis (FISH)
- In Situ Detection of CAR-T Cells & Oncolytic Viruses
- CAR-T/CAR-NK Target Assessment Service (ISH)
- ImmunoFISH Analysis (FISH+IHC)
- Splice Variant Analysis (FISH)
- Telomere Length Analysis (Q-FISH)
- Telomere Length Analysis (qPCR assay)
- FISH Analysis of Microorganisms
- Neoplasms FISH Analysis
- CARD-FISH for Environmental Microorganisms (FISH)
- FISH Quality Control Services
- QuantiGene Plex Assay
- Circulating Tumor Cell (CTC) FISH
- mtRNA Analysis (FISH)
- In Situ Detection of Chemokines/Cytokines
- In Situ Detection of Virus
- Transgene Mapping (FISH)
- Transgene Mapping (Locus Amplification & Sequencing)
- Stable Cell Line Genetic Stability Testing
- Genetic Stability Testing (Locus Amplification & Sequencing + ddPCR)
- Clonality Analysis Service (FISH)
- Karyotyping (G-banded) Service
- Animal Chromosome Analysis (G-banded) Service
- AAV Biodistribution Analysis (RNA ISH)
- Molecular Karyotyping (aCGH)
- Droplet Digital PCR (ddPCR) Service
- Digital ISH Image Quantification and Statistical Analysis
- SCE (Sister Chromatid Exchange) Analysis
- Biosample Services
- Histology Services
- Exosome Research Services
- In Vitro DMPK Services
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In Vivo DMPK Services
- Pharmacokinetic and Toxicokinetic
- PK/PD Biomarker Analysis
- Bioavailability and Bioequivalence
- Bioanalytical Package
- Metabolite Profiling and Identification
- In Vivo Toxicity Study
- Mass Balance, Excretion and Expired Air Collection
- Administration Routes and Biofluid Sampling
- Quantitative Tissue Distribution
- Target Tissue Exposure
- In Vivo Blood-Brain-Barrier Assay
- Drug Toxicity Services
Protocol for Preparing HPLC Standards for Drug Quantification
GUIDELINE
High-performance liquid chromatography (HPLC) is a cornerstone technique in pharmaceutical analysis for quantifying drugs and their metabolites. The use of HPLC standards is crucial for achieving accurate and precise results. This protocol describes the preparation of drug stock solutions to prepare standards for the quantitation of drugs using reversed-phase HPLC or ultraperformance liquid chromatography (UPLC) with ultraviolet/Visible wavelength (UV/Vis) detection.
METHODS
For 1.0 mg/mL ultimate stock
- Weigh out 2-7 mg drug powder with balance on the 4th floor using an antistatic micro spatula into an 8 mL amber glass vial.
- Carefully add enough HPLC-grade methanol to the vial to create a 1.0 mg/mL ultimate stock.
- Cap the vial and vortex for 10 seconds at high speed to mix and ensure all drug powder is dissolved in the methanol.
- Place in the sonicating bath for 1 minute to help dissolve any remaining drug then vortex 10 seconds at high speed to mix.
- Label vial with: drug name; 1 mg/ml in methanol; date; your initials.
For 200 ug/mL working stock
- Carefully add 800 μL HPLC grade Methanol to a 4 mL glass amber vial.
- Carefully add 200 μL of 1.0 mg/mL drug stock to the methanol in the 4 mL vial.
- Pipette the solution up and down several times to rinse the tip after adding to methanol.
- Cap and vortex for 10 seconds at high speed to mix.
- Label vial with: drug name; 200 μg/ml in methanol; date; your initials.
- Tightly wrap the caps of both vials with parafilm to ensure they are tightly sealed; store each at -80°C.
Creative Bioarray Relevant Recommendations
- Creative Bioarray provides various in vitro ADME/PK services, including high-throughput ADME screening, in vitro binding, in vitro metabolism, in vitro permeability, and transporter assays.
- We also provide in vivo drug metabolism and pharmacokinetic (DMPK) services to support drug development studies of in vivo absorption, distribution, metabolism, and excretion of drug candidates. Our in vivo DMPK services cover a comprehensive range of different animal studies in several species.
NOTES
- Saturate the pipette tip thoroughly by pipetting up and down twice before adding to the vial to avoid dripping.
- Ensure all glassware and instruments are clean to avoid contamination.
- Maintain consistent temperature and solvent composition.
- Document all procedures and calculations meticulously for reproducibility.
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For research use only. Not for any other purpose.