About Us
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Cell Services
- Cell Line Authentication
- Cell Surface Marker Validation Service
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Cell Line Testing and Assays
- Toxicology Assay
- Drug-Resistant Cell Models
- Cell Viability Assays
- Cell Proliferation Assays
- Cell Migration Assays
- Soft Agar Colony Formation Assay Service
- SRB Assay
- Cell Apoptosis Assays
- Cell Cycle Assays
- Cell Angiogenesis Assays
- DNA/RNA Extraction
- Custom Cell & Tissue Lysate Service
- Cellular Phosphorylation Assays
- Stability Testing
- Sterility Testing
- Endotoxin Detection and Removal
- Phagocytosis Assays
- Cell-Based Screening and Profiling Services
- 3D-Based Services
- Custom Cell Services
- Cell-based LNP Evaluation
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Stem Cell Research
- iPSC Generation
- iPSC Characterization
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iPSC Differentiation
- Neural Stem Cells Differentiation Service from iPSC
- Astrocyte Differentiation Service from iPSC
- Retinal Pigment Epithelium (RPE) Differentiation Service from iPSC
- Cardiomyocyte Differentiation Service from iPSC
- T Cell, NK Cell Differentiation Service from iPSC
- Hepatocyte Differentiation Service from iPSC
- Beta Cell Differentiation Service from iPSC
- Brain Organoid Differentiation Service from iPSC
- Cardiac Organoid Differentiation Service from iPSC
- Kidney Organoid Differentiation Service from iPSC
- GABAnergic Neuron Differentiation Service from iPSC
- Undifferentiated iPSC Detection
- iPSC Gene Editing
- iPSC Expanding Service
- MSC Services
- Stem Cell Assay Development and Screening
- Cell Immortalization
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ISH/FISH Services
- In Situ Hybridization (ISH) & RNAscope Service
- Fluorescent In Situ Hybridization
- FISH Probe Design, Synthesis and Testing Service
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FISH Applications
- Multicolor FISH (M-FISH) Analysis
- Chromosome Analysis of ES and iPS Cells
- RNA FISH in Plant Service
- Mouse Model and PDX Analysis (FISH)
- Cell Transplantation Analysis (FISH)
- In Situ Detection of CAR-T Cells & Oncolytic Viruses
- CAR-T/CAR-NK Target Assessment Service (ISH)
- ImmunoFISH Analysis (FISH+IHC)
- Splice Variant Analysis (FISH)
- Telomere Length Analysis (Q-FISH)
- Telomere Length Analysis (qPCR assay)
- FISH Analysis of Microorganisms
- Neoplasms FISH Analysis
- CARD-FISH for Environmental Microorganisms (FISH)
- FISH Quality Control Services
- QuantiGene Plex Assay
- Circulating Tumor Cell (CTC) FISH
- mtRNA Analysis (FISH)
- In Situ Detection of Chemokines/Cytokines
- In Situ Detection of Virus
- Transgene Mapping (FISH)
- Transgene Mapping (Locus Amplification & Sequencing)
- Stable Cell Line Genetic Stability Testing
- Genetic Stability Testing (Locus Amplification & Sequencing + ddPCR)
- Clonality Analysis Service (FISH)
- Karyotyping (G-banded) Service
- Animal Chromosome Analysis (G-banded) Service
- AAV Biodistribution Analysis (RNA ISH)
- Molecular Karyotyping (aCGH)
- Droplet Digital PCR (ddPCR) Service
- Digital ISH Image Quantification and Statistical Analysis
- SCE (Sister Chromatid Exchange) Analysis
- Biosample Services
- Histology Services
- Exosome Research Services
- In Vitro DMPK Services
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In Vivo DMPK Services
- Pharmacokinetic and Toxicokinetic
- PK/PD Biomarker Analysis
- Bioavailability and Bioequivalence
- Bioanalytical Package
- Metabolite Profiling and Identification
- In Vivo Toxicity Study
- Mass Balance, Excretion and Expired Air Collection
- Administration Routes and Biofluid Sampling
- Quantitative Tissue Distribution
- Target Tissue Exposure
- In Vivo Blood-Brain-Barrier Assay
- Drug Toxicity Services
Protocol for Chromosome Preparation in Human Cancer Cells
GUIDELINE
Corresponding cell lines have now been established for most cancers. Cancer cells cultured in vitro mostly belong to the continuous cycle cells, which can be used to prepare chromosomes. The conventional method for preparing animal and human chromosomes at home and abroad is the air-drying method.
METHODS
- Vigorous growing cells are collected and blown to disperse. Pretreat by adding colchicine to reach a final concentration of 1 μg/ml.
- After 6-8 hours of pretreatment, the cells are centrifuged at 800 rpm for 7 min and the supernatant is removed.
- The cells are resuspended by adding 1 ml of 0.075M KCl solution and blowing gently. Then makeup 7 ml of KCl solution and hypotonic for 20 min at room temperature.
- Add 3-4 drops of Carnoy fixative and centrifuge at 800 rpm for 7 min to remove supernatant.
- Add 3 ml of fixative slowly along the wall of the tube. After 2 min, blow gently with a pipette to disperse the cells. After fixation for 10 min, centrifuge to remove supernatant.
- Add 3 ml of newly prepared fixative and blow well, fix for 10 min, and centrifuge at 800 rpm to remove supernatant. Repeat the procedure.
- After removing the supernatant, 0.2-0.5 ml of fixative is left, and it is blown with a pipette to make a cell suspension.
- Pipeted a drop of cell suspension on an ice-cold slide (soaked in 80% ethanol and refrigerated at 0-4°C), blowing it away and overheating it for about 5 s.
- Blow-dried by a fan. Place in a staining vat with Giemsa staining solution, stain for 20 min, wash with water, blow-dry, and examine microscopically.
Creative Bioarray Relevant Recommendations
- In cancer research, cell lines derived from tumors are commonly used as models, because they are presumed to carry the genomic and epigenomic alterations that arise in tumors. Creative Bioarray offers 2,000+ tumor cells from humans and animals in stock, the details are as follows.
Product Types | Description |
Human Tumor Cells | We provide our customers with more than 1,000 human tumor cells from different organs and tissues. |
Animal Tumor Cells | Our animal tumor cells cover dozens of different animal species including mice, rats, bovines, canines, etc. |
Tumor Cell Media | Our cell culture media are designed to ensure continuous tumor cell proliferation in vitro. |
Tumor Cell Panels | Our tumor cell panels are based on key components of cell signaling pathways or cancer genes. |
NOTES
A method of preparing chromosomes as opposed to the sectioning and pressing methods used in the past. The cell suspension is first prepared, and then the suspension is dropped on a slide so that the cells and chromosomes are dispersed and adhere to the slide, which is dried naturally or by a blower and is ready for staining and examination.
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For research use only. Not for any other purpose.