Capsaicin-Induced Dermal Blood Flow Model

Creative Bioarray has developed the capsaicin-induced dermal blood flow model as a tool to assess the effectiveness of potential drug candidates in the treatment of migraine. This model is based on the observation that capsaicin, a compound found in chili peppers, can induce vasodilation and increase blood flow in the skin, mimicking the vascular changes seen in migraine attacks. By measuring the changes in dermal blood flow before and after the administration of a test compound, researchers can gain insights into its potential to modulate the vascular response associated with migraine.

Migraine, a chronic and debilitating neurovascular disorder, affects millions of individuals worldwide. The etiology of migraine has been the subject of extensive research, leading to the development of the "trigeminovascular theory" as a prevailing hypothesis. This theory posits that the trigeminovascular system (TGVS) plays a central role in the pathophysiology of migraine. Activation of the TGVS triggers cranial vasodilation through the release of vasoactive neuropeptides, including calcitonin gene-related peptide (CGRP).

One approach to investigate the role of CGRP in migraine pathophysiology and to assess the engagement of CGRP-blocking therapeutics is the utilization of the "capsaicin model." Capsaicin, the active component of hot chili peppers, activates the transient receptor potential vanilloid type 1 (TRPV1) receptor. Binding of capsaicin to TRPV1 stimulates the release of various bioactive substances, including CGRP. When applied to peripheral tissues like the skin, the resulting vasodilation can be readily observed, offering a clear endpoint for studying the effects of CGRP and evaluating the efficacy of CGRP-targeted therapies.

Fig. 1 Activation of the TRPV1 receptor by capsaicin causes CGRP release. (Buntinx et al. 2015)

This model provides a controlled and reproducible environment for screening and evaluating compounds that may have therapeutic benefits for migraine patients, helping to identify promising leads for further development.

Our Capsaicin-Induced Dermal Blood Flow Model

• Available Animal

Rat

• Modeling Method

At Creative Bioarray, we mimic a migraine by topically applying a small amount of capsaicin on the back of animals.

• Endpoints

• Dermal blood flow (DBF)
• Serum, brain, other tissue collection
• Histology analysis
• qPCR or Western blot
• Other customized endpoints: available upon requests.

Additionally, we also provide another migraine model that maybe you are interested in:

• Nitroglycerin (NTG)-Induced Migraine Model

Quotation and Ordering

Creative Bioarray is proud to offer comprehensive preclinical in vivo contract drug discovery and development services, supported by a team of highly skilled scientists and state-of-the-art facilities. Our commitment to excellence is evident in every aspect of our operations, from the design of our experiments to the analysis of our data. Whether you are looking to screen potential drug candidates or optimize your lead compounds, we have the expertise and resources to meet your needs. If you are interested in our services, please feel free to contact us at any time or submit an inquiry to us directly.

Reference

1. Buntinx, L., et al. Development of anti-migraine therapeutics using the capsaicin-induced dermal blood flow model. Br J Clin Pharmacol, 2015;80(5):992-1000.

Explore Other Options

• Cancer-Induced Bone Pain (CIBP) Model
• Inflammatory Pain Models
• Migraine Models
• Neuropathic Pain Models
• Postoperative Pain (POP) Model

• Nitroglycerin (NTG)-Induced Migraine Model

For research use only. Not for any other purpose.