Three-Dimensional Cell Culture Model Utilization in Renal Carcinoma Cancer Stem Cell Research

Abstract

BACKGROUND: Specific 3D conditions of cancer cell lines have been optimized over last years, with growing significance of serum-free and xeno-free culturevariants. The choice of proper culture media enables cancer stem cells proliferation in primary and stable cell lines.

METHODS: To obtain renal cellcancer stem-like phenotype, we employed media dedicated for mesenchymal cells and adult stem cells. Developed RCC cell line 3D culturesystem enables effective drug testing, including tyrosine kinase inhibitor anti-cancer cell toxicity.

RESULTS: To induce formation of 3D spheroids by RCC cell lines, StemXvivo and NutriStem media must be used. Usage of laminin- or poly-D-lysine coated plates enhances also the formation of spheroids in 3D-promoting media. Seeding is optimal with Caki-1 or ACHN cell lines as well as 786-O or HKCSC cells. Our bio-mimic 3D RCC cell culture model promotes cell viability and stem-related gene expression including E-cadherin, N-cadherin, HIF1, HIF2, VEGF, Sox2, Pax2, and Nestin. 3D spheroid formation ability and spheroid volume increase are disturbed upon drug treatment. Untreated 3D structures reach ~100 μm in diameter at the end of 14-day long experiment. Sorter-based cell cycle analysis and Ki-67 staining should be conducted to verify specific toxicity.

CONCLUSION: We suggest that due to the more complex architecture 3D RCC culture is more relevant to investigate the in vivo-like tumor drug response.