Description
Porcine Hepatic Stellate Cells were transfected with SV40LT expressing lentiviral particles. These primary cells go into senescence after the 2nd passage while the Immortalized Porcine Hepatic Stellate Cells go beyond 30 passages.
Recommended Medium
SuperCult® Immortalized Porcine Hepatic Stellate Cell Medium (Cat No.: CM-I2066Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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What research applications are Immortalized Porcine Hepatic Stellate Cells suitable for?
Immortalized Porcine Hepatic Stellate Cells are ideal for various research applications, including:
Liver fibrosis and cirrhosis studies.
Hepatic stellate cell activation and signaling pathways.
Drug discovery and toxicity testing.
Liver metabolism and function analysis.
Cell-cell interaction in liver microenvironments.