Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC)

CSC-I9282L

A spontaneously immortalized cell clone arisen from isolated adipose-derived stem cells (ASCs), the Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC) display a typical fibroblastoid morphology. The cell line stably expresses the stem cell markers Sca-1, c-Kit/CD117, nestin, nucleostemin, CD44, and CD106, but is negative for the embryo stem cell markers Nanog, Oct4, and Sox2. The cells were endowed with multipotency and can be induced to differentiate toward osteogenic, chondrogenic, adipogenic, and cardiogenic phenotypes.It displays a normal karyotype and stable telomeres, neither proliferates when plated in soft agar nor gives rise to tumors when injected subcutaneously in NOD/SCID-g null mice. The cell line is thus recommended as a valuable tool for a number of applications in ex vivo tissue engineering to study the differentiation mechanisms involved in tissue repair, as well as a model for both pharmacological and toxicological studies.

Mus musculus

Adipose tissue

Adherent

Fibroblast-like

Spontaneous immortalization

For Research Use Only

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.

Dry Ice.

CIK-HT003 HT® Lenti-SV40T Immortalization Kit

1) RT-PCR and Cytofluorimetry were performed on the cDNA from the cells to confirm the expression of stem cell markers Sca-1, c-kit, Islet 1, nestin, and nucleostemin.
2) Cells after differentiation were specifically stained with alizarin red, adipo-red and Alcian blue, and the tissue-specific collagen II antibodies, respectively to confirm osteogenic, adipogenic, and chondrogenic phenotypes the cells’ multipotency.
3) G-banding analysis for macroscopic chromosome alterations over time compared with normal mouse karyotypes.

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