Our Promise to You

Guaranteed product quality, expert customer support

Our Promise to You

ONLINE INQUIRY

Immortalized Chicken Skeletal Muscle Cells-SV40

Cat.No.: CSC-I9234L

Species: Chicken

Source: Skeletal Muscle

Culture Properties: Adherent

  • Specification
  • Q & A
  • Customer Review
Cat.No.
CSC-I9234L
Description
Immortalized Chicken Skeletal Muscle Cells-SV40 have been obtained immortalizing Chicken Primary Skeletal Muscle Cells with Lenti-SV40 Lentivirus. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 3rd passage while the SV40tranduced cells go beyond 30 passages.
Species
Chicken
Source
Skeletal Muscle
Recommended Medium
SuperCult® Immortalized Chicken Skeletal Muscle Cell Medium (Cat No.: CM-I9234L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Quality Control
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What are Immortalized Chicken Skeletal Muscle Cells-SV40?

Immortalized Chicken Skeletal Muscle Cells-SV40 are chicken muscle cells that have been genetically modified with SV40 large T antigen to go beyond 20 passages. This modification enables them to overcome the normal senescence seen in primary cells, allowing for extensive study and experimentation.

Why should I use Immortalized Chicken Skeletal Muscle Cells in my research?

These cells are invaluable for consistent, long-term studies in muscle biology. They provide a stable, reproducible platform for applications such as muscle physiology, regenerative medicine research, drug testing, and genetic studies, ensuring more reliable and robust data.

How were these cells immortalized?

The immortalization process involves introducing the SV40 large T antigen into chicken skeletal muscle cells. This gene promotes continuous cell division while maintaining the essential properties of the muscle cells, facilitating extended research capabilities.

What sort of research applications are Immortalized Chicken Skeletal Muscle Cells suitable for?

Immortalized Chicken Skeletal Muscle Cells-SV40 (Cat No.: CSC-I9234L) are perfect for a variety of applications, including muscle development and regeneration studies, disease modeling for myopathies, drug screening and toxicity testing, and functional genomic studies focused on muscle-specific genes and pathways.

What advantages do these immortalized cells offer over primary chicken muscle cells?

Immortalized cells provide a constant, renewable source of cells with uniform characteristics, reducing variability typically associated with primary cells. They facilitate long-term experiments without the limitations of primary cell lifespan and donor variability.

What are the recommended culture conditions for these cells?

Our Immortalized Chicken Skeletal Muscle Cells come with comprehensive culture guidelines, specifying the appropriate media, supplements, and environmental conditions required for optimal growth and maintenance, ensuring the best experimental outcomes.

Ask a Question

Write your own review

For research use only. Not for any other purpose.