WSU-FSCCL

Cat.No.: CSC-C0611

Species: Human

Source: B cell lymphoma

Morphology: round to polymorph cells growing singly in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0611

Description

Established from the peripheral blood of a 37-year-old white man with low-grade follicular small cleaved cell lymphoma (B-NHL, stage IVB) after chemotherapy and transformation to small cell non-cleaved non-Hodgkin′s lymhoma in 1989; described to carry t(14;18) and t(8;11) with BCL2 and MYC rearrangements, respectively

Species

Human

Source

B cell lymphoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

round to polymorph cells growing singly in suspension

Karyotype

Human hyperdiploid karyotype with 4% polyploidy; 47(46-50)<2n>XY, -13, +2mar, add(1)(p34), del(6)(q25), t(8;11)(q24.3;q24), i(13q), t(14;18)(q32;q21); t(14;18) effects IGH@-BCL2 (IGH-BCL2) rearrangement

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why is FISH better than karyotyping?

While conventional karyotyping provides a comprehensive view of the genome, FISH can detect cryptic or submicroscopic genetic abnormalities and identify recurrent genetic abnormalities in non-dividing cells.

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