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UCSD-AML1

Cat.No.: CSC-C0672

Species: Human

Source: acute myeloid leukemia

Morphology: single cells in suspension, a few cells are lightly adherent

Culture Properties: suspension

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Cat.No.
CSC-C0672
Description
Established from the bone marrow of a 73-year-old woman with acute myeloid leukemia at relapse in 1989; cells were described to be strictly cytokine-dependent (GM-CSF, IL-6, or M-CSF) and to carry the translocations t(3;3)(q21;q26) and t(12;22)(p13;q12) leading to MECOM (EVI1) overexpression and to ETV6-MN1 (TEL-MN1) fusion gene, respectively
Species
Human
Source
acute myeloid leukemia
Recommended Medium
80% RPMI-1640 + 20% h.i. FBS + 10 ng/ml GM-CSF
Culture Properties
suspension
Morphology
single cells in suspension, a few cells are lightly adherent
Karyotype
Human flat-moded hypodiploid karyotype with 10% polyploidy; 45(42-45)<2n>XX,-7, t(3;3)(q21;q26), t(12;22)(p13;q12); carries t(3;3) and t(12;22) effecting MN1 and ETV6/MN1 rearrangements, respectively; precisely matches published karyotype
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, cyCD68 +, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
How are STRs detected by PCR?

Historically, STRs have been genotyped using polymerase chain reaction (PCR) and gel electrophoresis. In such cases, PCR is performed using primers complementary to unique sequences flanking the STR. The PCR product is then run on a capillary electrophoresis gel to determine its size.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

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30 June 2023


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