SUP-HD1

Cat.No.: CSC-C0579

Species: Human

Source: Hodgkin lymphoma

Morphology: round cells growing singly or in small clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0579

Description

Established from a 37-year-old man at second (refractory/terminal) relapse of Hodgkin lymphoma (nodular sclerosing -> lymphocyte depleted/stage IIISA -> stage IV) after both combined chemo- and radiotherapy in 1987

Species

Human

Source

Hodgkin lymphoma

Recommended Medium

SuperCult® McCoys 5a Medium and 10% h.i. FBS.

Culture Properties

suspension

Morphology

round cells growing singly or in small clumps in suspension

Karyotype

Human highly rearranged hyperdiploid karyotype with 4% polyploidy - 48(44-48)<2n>X, -Y, +5, +8, +9, -13, +19, +mar, der(1)t(1;1)(p34;q12.2), del(2)(p23), ins(2;8)t(p2?3;??), add(4)(p15), add(4)(q23), add(5)(p1?1), i(5p), der(5)t(5;15)(p10;p10), del(6)(p23

Quality Control

Mycoplasma: contamination was eliminated with MRA (Mycoplasma Removal Agent), then negative in microbiological culture, PCR assays
Immunology: CD2 -, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD25 +, CD30 -, CD34 -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, H

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is an in situ probe?

In situ hybridization indicates the localization of gene expression in their cellular environment. A labeled RNA or DNA probe can be used to hybridize to a known target mRNA or DNA sequence within a sample. This labeled RNA or DNA probe can then be detected by using an antibody to detect the label on the probe.

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