SU-DHL-6

Cat.No.: CSC-C0577

Species: Human

Source: B cell lymphoma

Morphology: small single cells and in clusters, in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0577

Description

Established from the peritoneal effusion of a 43-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse, mixed small and large cell type; cell line carries EZH2 Y641N mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell)

Species

Human

Source

B cell lymphoma

Recommended Medium

80-85% RPMI-1640 + 15-20% h.i. FBS

Culture Properties

suspension

Morphology

small single cells and in clusters, in suspension

Karyotype

Human hyperdiploid karyotype with 2% polyploidy - 47(42-48)<2n>X, -Y, +6, +7, del(4)(q23), del(6)(p21.3p22.2), i(6p), del(7)(q?22q?32), der(8)t(8;9)(q24;p13), der(9)t(8;19;9)(q24;q13;p13), dup(11)(q24q25), t(14;18)(q32;q21), der(22)t(?7;22)(?q32;p11) - si

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are FISH probes for cancer?

In FISH testing, pieces of single-strand DNA (called DNA probes) are sent to find corresponding stretches of DNA from tumor cells sampled from the body. The probes, marked with a fluorescent dye, attach to those corresponding cells—that process is called hybridization.

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