SEWA

Cat.No.: CSC-C1115

Species: Mouse

Source: osteogenic sarcoma

Morphology: round cells growing singly or in clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C1115

Description

Established from a polyoma virus-induced osteogenic sarcoma in an A.SW mouse; cells were converted into ascitic form growing in suspension by serial intraperitoneal inoculation into genetically compatible mice; described to have lost double minute chromosomes which were still present in the original tumor cell line, but reoccur when retransplanted into mice

Species

Mouse

Source

osteogenic sarcoma

Recommended Medium

90-95% RPMI-1640 + 5-10% h.i. FBS

Culture Properties

suspension

Morphology

round cells growing singly or in clumps in suspension

Karyotype

Murine flat-moded near-diploid karyotype - 36-44<2n> - dmin rare or absent

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Viruses: PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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