SET-2

Cat.No.: CSC-C0608

Species: Human

Source: essential thrombocythemia

Morphology: mostly single cells in suspension, some cells grow loosely adherent, 1-3% giant cells

Culture Properties: suspension

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Cat.No.

CSC-C0608

Description

Established from the peripheral blood of a 71-year-old woman with essential thrombocythemia at megakaryoblastic leukemic transformation in 1995; cells were described to carry the JAK2 V617F mutation

Species

Human

Source

essential thrombocythemia

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

mostly single cells in suspension, some cells grow loosely adherent, 1-3% giant cells

Karyotype

Human flat-moded near-diploid karyotype with 8% polyploidy; 46-47<2n>XX, +13, -14, +21, +mar, add(4)(p15), der(5)t(5;19)(q11;q13), der(7)t(7;14)(q31;q11), del(9)(p11p24)ins(9;?21)(p11;p11p12), add(9)(q11)t(9;22)(p24;q12), der(13)del(13)(q11-q13)t(13;21)(q

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD7 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD38 +, CD42 -, CD71 +, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the limitations of karyotyping?

Conventional karyotyping is limited by its inability to identify cryptic abnormalities, complex aberrations, and marker chromosomes accurately.

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