SER-W3

Cat.No.: CSC-C1387

Species: Rat

Source: rat Sertoli cells

Morphology: epitheloid cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C1387

Description

Sertoli cells dissected from normal testis from 17-day-old Wistar rat and immortalized with SV40 large-T antigen; cells were described as displaying morphological and functional features of Sertoli cells (phagocytosis, cisplatin sensitivity, transferrin secretion)

Species

Rat

Source

rat Sertoli cells

Recommended Medium

90% DMEM + 10% h.i.FBS

Culture Properties

monolayer

Morphology

epitheloid cells growing in monolayers

Karyotype

Rat flat-moded hypotetraploid karyotype - 70-78<4n>XXYY/XXY - supernumerary r/acf present in most cells

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.