RV-C2

Cat.No.: CSC-C6166X

Species: Mouse

Source: hybridoma

Morphology: round single cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C6166X

Description

Described as secreting a mouse monoclonal antibody (IgG2b) reacting with fetal, neonatal and adult cardiac fibers in immunofluorescence and with rat cardiac fetal and adult troponin T in Western blotting; in immunofluorescence this antibody stains some rat fetal and neonatal skeletal muscle fibers which express fetal cardiac troponin T as also shown by the Western blotting of rat fetal and neonatal skeletal muscle; this cell line was produced by fusing NSO myeloma cells with spleen cells from a Balb/c mouse that had been immunized with a myofibrillar preparation from hypothyroid rabbit ventricle

Species

Mouse

Source

hybridoma

Recommended Medium

80% DMEM + 20% horse serum.

Culture Properties

suspension

Morphology

round single cells in suspension

Karyotype

Murine hypertetraploid/pentaploid karyotype - 88-99<4n> - 2 centric fusion markers and 1-2 dmin present in most cells

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG2b monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% horse serum, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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