PSI-2

Cat.No.: CSC-C1082

Species: Mouse

Source: embryo - NIH-3T3 derivative

Morphology: adherent fibroblastoid cells growing as monolayers

Culture Properties: monolayer

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C1082

Description

Subclone of the Swiss mouse embryo cell line NIH-3T3; PSI-2 cells are infected with a replication deficient MLV virus; cells can be used as "packaging cell line"; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS)

Species

Mouse

Source

embryo - NIH-3T3 derivative

Recommended Medium

90% DMEM + 10% h.i.FBS

Culture Properties

monolayer

Morphology

adherent fibroblastoid cells growing as monolayers

Karyotype

Murine hypotetraploid karyotype - centric fragment present at 74% - ring present at 48% - 68/69(64-74)<4n> - a submetacentric marker arising by centric fusion present at 52%

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Ask a Question