PAB-100

Cat.No.: CSC-C6193X

Species: Mouse

Source: hybridoma

Morphology: single cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C6193X

Description

Established by fusion of NS-1 myeloma cells and spleen cells from a female BALB/c mouse immunized with SVT-2, an SV40-transformed BALB/c mouse cell line; cells reportedly secrete an IgG1 antibody recognizing denaturation-sensitive antigenic determinants unique to large T antigen

Species

Mouse

Source

hybridoma

Recommended Medium

80% DMEM + 20% h.i.FBS + non-essential amino acids

Culture Properties

suspension

Morphology

single cells growing in suspension

Karyotype

Predominantly mouse chromosomal morphology with 3 iso-chromosome markers present in most cells - 140(134-155)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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