P-388D1(IL-1)

Cat.No.: CSC-C2044

Species: Mouse

Source: monocytes-macrophages

Morphology: adherent elliptical and round loosely adherent cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C2044

Description

Established from the ascites of a DBA/2 mouse with a lymphoid neoplasm induced by methylcholanthrene and subsequently enriched for adherent (monocyte-macrophage) cells by selection (yielding this IL1-producing subclone); reported to produce IL-1 after stimulation with LPS or PMA, to phagocytize latex particles and to synthesize lysozyme

Species

Mouse

Source

monocytes-macrophages

Recommended Medium

80% RPMI-1640 + 20% horse serum + 3.5 g/L glucose

Culture Properties

monolayer

Morphology

adherent elliptical and round loosely adherent cells growing in monolayers

Karyotype

Murine hypertriploid karyotype with 8% polyploidy - 61(57-65)<3n> - 5-8 centric fusion markers

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 15% horse serum, 5% FBS, 10% DMSO at about 2-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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