OMEGA-E

Cat.No.: CSC-C1083

Species: Mouse

Source: NIH-3T3 (Swiss mouse embryo) derivative-retroviral packaging cell line

Morphology: cells grow as adherent fibroblastic monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C1083

Description

Derived from NIH-3T3 cells; high titer ecotropic helper-free packaging cell line; generated with separate gagpol and ecotropic env expression constructs with minimal sequence overlap and decreased sequence homology achieved by "codon wobbling"; prior to use in nude mice, cells need to be subcloned; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS)

Species

Mouse

Source

NIH-3T3 (Swiss mouse embryo) derivative-retroviral packaging cell line

Recommended Medium

90% DMEM + 10% NCS

Culture Properties

monolayer

Morphology

cells grow as adherent fibroblastic monolayers

Karyotype

Murine hypertriploid karyotype - 71(63-73)<3n> - 1-2 dm (24% cells)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Cytogenetics: murine hypertriploid karyotype - 71(63-73) - 1-2 dm (24% cells)
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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