NC-37

Cat.No.: CSC-C9504L

Species: Human

Source: B cell; B lymphoblast

Morphology: lymphoblast

Culture Properties: suspension

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Cat.No.

CSC-C9504L

Description

Species: human - male, 34 years old, Caucasian
Isoenzyme: G6PD, B
Note: the cells are EBNA positive(EBNA+) and surface immunoglobulin negative(slg-); the lines carries Epstein-Barr virus(EBV) and contains 60 copies of the EBV genome per cell

Species

Human

Source

B cell; B lymphoblast

Recommended Medium

90% RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3 + 10% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblast

STR DNA Profile

D3S1358: 15,17
vWA: 16,17
FGA: 26,28
Amelogenin: X,Y
TH01: 6,9.3
TPOX: 10,11
CSF1P0: 10,12
D5S818: 11,13
D13S317: 12,13
D7S820: 9,11

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule
Shipping Condition: Room Temperature

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why is EDTA added to trypsin in cell culture?

EDTA enhances the cleavage ability of trypsin to help weaken cell adhesion in cell suspensions. In some formulations, phenol red is added as a pH indicator. Among its applications, Trypsin-EDTA can be used to generate single-cell lines for stem cell research.

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