MN-60

Cat.No.: CSC-C0295

Species: Human

Source: B cell leukemia

Morphology: single, round to polygonal cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0295

Description

Established from the peripheral blood of a 20-year-old man with acute B cell leukemia (ALL FAB L3) in partial remission in 1981

Species

Human

Source

B cell leukemia

Recommended Medium

90% Hams F10 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, round to polygonal cells in suspension

Karyotype

Human near diploid karyotype - 46(45-47)<2n>XY, dup(1)(q21q41), del(6)(q21), t(8;14)(q24;q32), i(13q)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +
Virus

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x106 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What happens if cells become too confluent?

Cells may be stressed and undergo cell death due to competition for resources and overcrowding. Generally, you should avoid letting your cells become over-confluent because they'll likely start to die off (sometimes very quickly).

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