MHH-CALL-4

Cat.No.: CSC-C0396

Species: Human

Source: B cell precursor leukemia

Morphology: single, round cells growing in suspension, partly in clusters

Culture Properties: suspension

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Cat.No.

CSC-C0396

Description

Established from the peripheral blood of a 10-year-old Caucasian boy with acute lymphoblastic leukemia (pre B-ALL) at diagnosis in 1993

Species

Human

Source

B cell precursor leukemia

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

single, round cells growing in suspension, partly in clusters

Karyotype

Human hypodiploid karyotype with 4% polyploidy - 45(42-46)<2n>X, -Y, r(12)(qter;p13-pter)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 +, CD13 +, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Viruses: ELISA:

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is flow cytometry leukemia?

Leukemia is diagnosed based on clinical information and laboratory testing results. Flow Cytometry (FCM) is a technology capable of providing rapid multi-parameter analysis of single cells or particles.

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