MC-116

Cat.No.: CSC-C0238

Species: Homo sapiens (human)

Morphology: round to elongated, lymphoblastoid, single or clustered cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0238

Description

Established from the pleural effusion of a patient with B cell lymphoma (undifferentiated lymphoma); cells were described to be EBV-negative, to carry the t(8;14) and to express mRNA of the proto-oncogene BCL2

Species

Homo sapiens (human)

Tissue of Origin

Ascites

Recommended Medium

80-85% RPMI-1640 + 15-20% h.i. FBS

Culture Properties

suspension

Morphology

round to elongated, lymphoblastoid, single or clustered cells in suspension

Karyotype

Human near diploid karyotype with 1.5% polyploidy - 46(37-46)<2n>XY, dup(1)(q21q32), t(8;14)(q24;q32), del(10)(q23)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 -, HLA-DR +, sm/cyIgM +, sm/cyIgG -, sm/cykappa -, sm/cylambda +
Viruses: ELISA: reverse transcriptase

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the difference between RPMI and RPMI 1640?

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.

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