LAMA-87

Cat.No.: CSC-C0368

Species: Human

Source: chronic myeloid leukemia in blast crisis

Morphology: round cells growing singly or in small clusters in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0368

Description

The parental cell line LAMA-84 has been established from the peripheral blood of a 29-year-old woman with chronic myeloid leukemia (CML) in blast crisis; subclone LAMA-87 was obtained after subcutaneous transplantation of LAMA-84 cells into estrone-treated nude mice; cells are described to carry t(9;22) leading to BCR-ABL1 e14-a2 (b3-a2) fusion gene

Species

Human

Source

chronic myeloid leukemia in blast crisis

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

round cells growing singly or in small clusters in suspension

Karyotype

Human flat-moded hypotriploid karyotype - 60-64<3n>XX, -X, -2, -3, -7, +8, -10, -13, -14, -15, -16, -18, +19, -20, +22, der(9)t(9;22)(q34;q11)x2, del(12)(p12), del(12)(q14), add(19)(p13), add(?19)(p11), der(22)t(9;22)(q34;q11)x4 - four copies of Ph presen

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, HLA-DR -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is a sterile technique in biology?

In sterile technique, only sterile surfaces touch other sterile surfaces and exposure to the air is kept to a minimum. In the classroom, you often need to practice the sterile technique when you inoculate a pure culture of a microorganism into a fresh medium.

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