L-82

Cat.No.: CSC-C0600

Species: Human

Source: anaplastic large cell lymphoma

Morphology: large round cells growing singly in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0600

Description

Established from the pleural effusion of a 24-year-old woman with recurrent anaplastic large cell lymphoma (ALCL); cells were described to clonally derive from T-lineage lymphoid cells and to be tumorigenic in SCID mice

Species

Human

Source

anaplastic large cell lymphoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

large round cells growing singly in suspension

Karyotype

Human flat-moded karyotype with 4% polyploidy 72-77<3n>XX/XXX,+1,+2,+5,+7,+9,+10,-14,-15,+4-6mar; add(X)(q12), add(X)(q27), add(1)(p11), t(2;5)(p23;q35), der(2)t(2;5)(p23;q35), der(4)add(4)(p14q35), der(6)i(6)(q10)t(1;6)(q41;p24), add(7)(q22), add(8)(q24)

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cy/smCD3 -, CD4 +, CD5 +, CD6 -, CD7 -, CD8 -, CD11b -, CD13 -, CD14 -, CD15 -, CD19 -, CD20 -, CD25 +, CD30 +, CD34 -, CD71 +, TRCalpha/beta -, TCRgamma/delta -
Viruses: PCR: EBV -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Can cancer cells be karyotyped?

Cancer cells are typically characterized by complex karyotypes including both structural and numerical changes, with aneuploidy being a ubiquitous feature.

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