L-591

Cat.No.: CSC-C0603

Species: Human

Source: B lymphoblastoid cells

Morphology: round cells growing in loosely-attached clusters

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Cat.No.

CSC-C0603

Description

Established from the pleural effusion of a 31-year-old woman with Hodgkin lymphoma (nodular sclerosis; stage IVB) in 1982

Species

Human

Source

B lymphoblastoid cells

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Morphology

round cells growing in loosely-attached clusters

Karyotype

Human near-diploid karyotype with 6% polyploidy; 46(45-48)<2n>XX, add(7)(q32), der(14)t(9;14)(p13;q32); der(14) effects rearrangement of PAX5 which (untypically for HL) is upregulated

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD2 +, CD3 -, CD4 -, CD8 -, CD10 -, CD19 +, CD20 -, CD21 +, CD25 +, CD30 +, CD34 -, CD80 +, CD138 -
Viruses: PCR: EBV +, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How does the karyotype of a normal cell compare to that of a cancerous cell?

When a normal cell is karyotyped the number of chromosomes will be 46, and when a cancer cell is karyotyped the number of chromosomes can increase up to 75-80.

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