KYO-1

Cat.No.: CSC-C0602

Species: Human

Source: chronic myeloid leukemia in blast crisis

Morphology: round cells growing singly or in clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0602

Description

Established from the peripheral blood of a 22-year-old Japanese man with chronic myeloid leukemia (CML) in myeloid blast crisis in 1981

Species

Human

Source

chronic myeloid leukemia in blast crisis

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Culture Properties

suspension

Morphology

round cells growing singly or in clumps in suspension

Karyotype

Human flat-moded hypotetraploid karyotype; 83-90<4n>XX, -Y, -Y, +5, +8, -9, -9, +11, +11, +15, -16, -17, -17, -18, -18, +22, +22, +2mar; t(3;12)(p12.2;p11-12) x2, der(5)t(5;6)(p15;?q22)t(6;12)(q2?;?)ins(6;12)(q2?;?), del(6)(q22) x2, der(8)t(8;9)(q25;p11-1

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD13 -, CD14 -, CD19 -, CD33 +, CD34 -, CD41 -, CD42b -, CD71 +, CD235a +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What type of cells can be used for karyotyping?

Karyotype analysis is performed in cells undergoing cell division, or mitosis. Thus, only cells that are rapidly dividing (bone marrow or chorionic villus) or can be stimulated to divide in culture (peripheral blood lymphocytes, skin fibroblasts, and amniocytes) are used.

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