KI-JK

Cat.No.: CSC-C0676

Species: Human

Source: anaplastic large cell lymphoma

Morphology: round to polymorph cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0676

Description

Established in 1989 from the pleural effusion of a 15-year-old boy with Ki-1 positive lymphoma (anaplastic large cell lymphoma; ALCL); carries the translocation t(2;5) leading to NPM1-ALK fusion gene; cells were originally described to be EBV positive; however, viral analyses (PCR and western blot) at the Creative Bioarray established that the cells do not harbor EBV

Species

Human

Source

anaplastic large cell lymphoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

round to polymorph cells growing in suspension

Karyotype

Human flat-moded hypertriploid karyotype; 75-82 <3n>XXYY, +1, +1, +2, +3, +6, +7, +8, -9, +16, -15, -17, +19, +20, +21, +2-3mar, add(Y)(q11), del(1)(p34)x2, der(1)t(1;5)(p11;q11)t(5;2)(q35;p21), der(1;16)(q10;p10), i(1)(q10)x2, t(2;5)(p23;q35)x1-2, add(4)

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD4 -, CD14 -, CD15 +, CD25 +, CD30 +, CD34 -, CD71 +, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the three types of cell culture techniques?

Cells cultured in the lab can be classified into three different types, including primary cells, transformed cells, and self-renewing cells.

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