JL-1

Cat.No.: CSC-C2542

Species: Human

Source: epithelioid mesothelioma

Morphology: adherent, epithelial-like cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C2542

Description

Established from the untreated malignant epithelioid mesothelioma of the pleura of a 54-year-old man with asbestos cancer in 2003

Species

Human

Source

epithelioid mesothelioma

Recommended Medium

80% DMEM or NCTC-109 + 20% h.i.FBS + 2 mM L-glutamine

Culture Properties

monolayer

Morphology

adherent, epithelial-like cells growing in monolayers

Karyotype

Human flat-moded karyotype with 12 % polyploidy; 42-47<2n>XY, +5, +7, -10, -13, +20, der(1)t(1;15)(q11;q11), inv(3)(p1?q2?), der(5)t(5;16)(q11;?), add(10)(q25), der(11)t(10;11)(q21;q23), der(14)t(1;14)(q11;q31); sdl with add(9)(p11), del(16)(q11); karyoty

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the cells in mesothelioma?

Epithelioid, sarcomatoid, and biphasic are the three main types of mesothelioma cells.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Good

With readily available and viable tumor cells, we can start experiments immediately, eliminating the need for time-consuming and costly cell line establishment procedures.

23 Apr 2023

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