JK-1

Cat.No.: CSC-C0403

Species: Human

Source: chronic myeloid leukemia in blast crisis

Morphology: round single cells growing in suspension (cell pellet shows reddish color hemoglobin)

Culture Properties: suspension

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Cat.No.

CSC-C0403

Description

Established from the biopsy material of the shoulder tumor of a 62-year-old man with chronic myeloid leukemia (CML) in erythroid blast crisis in 1987; described to produce hemoglobin; cells are described to carry t(9;22) leadin to BCR-ABL1 e13-a2 (b2-a2) fusion gene

Species

Human

Source

chronic myeloid leukemia in blast crisis

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

round single cells growing in suspension (cell pellet shows reddish color hemoglobin)

Karyotype

Human hyperdiploid karyotype without significant polyploidy - 48(45-48)<2n>XY, +8, +22, t(1;7)(q10;q10), der(9)t(9;22)(q34;q11), der(22)t(9;22)(q34;q11)x2 - carries two Ph - resembles published karyotype

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 (+), CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 (+), CD42 (+), CD71 +, CD235a +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How does immunohistochemistry detect tumor markers?

IHC reveals more than a standard biopsy test. It enables pathologists to determine the exact type and subtype of cancer by looking for unique markers within cancer cells. Markers are identified using antibodies, a type of protein that locates and binds with antigens (which are the markers found on cells).

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