HC-1

Cat.No.: CSC-C0381

Species: Human

Source: hairy cell leukemia

Morphology: lymphoblastoid cells growing as single cells or in clumps, in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0381

Description

Spontaneously immortalized peripheral blood leukocytes from a 56-year-old white man with hairy cell leukemia (HCL) at diagnosis; cells were described to grow in soft agar, to be tumorigenic in irradiated nude mice and to be nagative for the HCL-associated mutation BRAF V600E

Species

Human

Source

hairy cell leukemia

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblastoid cells growing as single cells or in clumps, in suspension

Karyotype

Human near-tetraploid karyotype with 3% polyploidy - 92(89-92)<4n>XXYY, add(2)(p13->23) - differs from published karyotype

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda -
Viruses: ELISA:

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Does fetal bovine serum contain DNA?

FBS is the source of a large portion of the 167-bp DNA fragments after up to 24 h of cell culture.

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