HAL-01

Cat.No.: CSC-C0609

Species: Human

Source: B cell precursor leukemia

Morphology: single, round cells in suspension, some cells are slightly adherent

Culture Properties: suspension

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Cat.No.

CSC-C0609

Description

Established from the peripheral blood of a 17-year-old woman with acute lymphoblastic leukemia (ALL-L2) in 1990; contains the t(17;19)(q22;p13) TCF3-HLF (E2A-HLF) fusion gene

Species

Human

Source

B cell precursor leukemia

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, round cells in suspension, some cells are slightly adherent

Karyotype

Human near-diploid karyotype with 4% polyploidy; 46(43-48)<2n>XX, t(1;7)(p34;q21), t(17;19)(q22;p13); t(17;19) effects rearrangement of TCF3 (E2A) and HLF; matches published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, S

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Do tumor cells have mutated DNA?

Cells become cancerous after mutations accumulate in the various genes that control cell proliferation. According to research findings from the Cancer Genome Project, most cancer cells possess 60 or more mutations.

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