FKH-1

Cat.No.: CSC-C0613

Species: Human

Source: acute myelocytic leukemia

Morphology: mostly single, round cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0613

Description

Established in 1993 from the peripheral blood of a 61-year-old man with Philadelphia chromosome negative chronic myeloid leukemia (CML) with trilineage myelodysplasia at refractory leukemic transformation into acute myeloid leukemia (AML M4); carries t(6;9)(p23;q34) leading to DEK-NUP214 (DEK-CAN) fusion gene; cells do not respond to G-CSF, as originally described

Species

Human

Source

acute myelocytic leukemia

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

mostly single, round cells in suspension

Karyotype

Human hypodiploid karyotype with 4% polyploidy; 45(43-46)<2n>XY, -7, t(6;9)(p23;q34); carries t(6;9) effecting rearrangement of DEK with NUP214 (CAN); closely resembles published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD11b +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What stage is best for karyotyping?

For karyotyping the chromosomes must be isolated from cells in the metaphase, which is the stage of the cell cycle in which the chromosomes assume their characteristic condensed, discrete shape.

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