EFM-192C

Cat.No.: CSC-C0388

Species: Human

Source: breast carcinoma

Morphology: epitheloid adherent cells growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0388

Description

Established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; sister cell lines EFM-192A and -192B show differences in molecular aspects; erbB-2 gene is amplified and overexpressed

Species

Human

Source

breast carcinoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

monolayer

Morphology

epitheloid adherent cells growing as monolayers

Karyotype

Highly rearranged human hypohexaploid karyotype with 10% polyploidy - 122-138<6n>XXXXX/XXXXXX - related to EFM-192A and EFM-192 B

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin -
Viruses: EL

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How does physical contamination affect cell growth?

Physical contamination affects cell metabolism by affecting the biochemical components of the cell culture system.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Good growth

The cells grew well, which also facilitated my later experiments.

25 Feb 2022

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For research use only. Not for any other purpose.