EFM-192B

Cat.No.: CSC-C0386

Species: Human

Source: breast carcinoma

Morphology: epitheloid adherent cells growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0386

Description

Established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; cells were described to overexpress the erB-2 gene; sister cell lines EFM-192A and -192C show differences in molecular aspects

Species

Human

Source

breast carcinoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

monolayer

Morphology

epitheloid adherent cells growing as monolayers

Karyotype

Human heteroploid hypotriploid karyotype with 13% polyploidy - 62(58-62)<3n>XXX/XX, -X, +2, +4, +6, -7, -8, -8, -10, -11, -12, +14, +16, -17, -17, -17, -18, -18, -19, -20, -21, -22, +8mar, add(1)(p22-31), add(2)(q?35), del(3)(p22), add(5)(p15), del(6)(q25

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 -, cytokeratin-17 +, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Viruses: EL

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What does physical contamination in cell culture involve?

Physical contamination includes temperature, radiation, vibration, radiation (UV or fluorescence) and others.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Reduced contamination

With the help of the technicians, the contamination of my cells during the culture process was greatly reduced.

11 Feb 2022

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For research use only. Not for any other purpose.