EFM-19

Cat.No.: CSC-C0338

Species: Human

Source: breast carcinoma

Morphology: epitheloid, growing in clusters at first, then as monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0338

Description

Established from the pleural effusion of a 50-year-old Caucasian woman with breast carcinoma (ductal type, histological grading II) in 1979

Species

Human

Source

breast carcinoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

epitheloid, growing in clusters at first, then as monolayer

Karyotype

Human highly rearranged hypotriploid karyotype with 5.8% polyploidy - 62(58-65)<3n>X, der(X), apparent ch 17 nullisomy and preponderance of marker chromosomes, e.g. add(1)(p11), idel(1)(q11q?22), i(1p), i(2p), add(4)(p11), add(4)(p11), etc. - resembes ori

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What do I need to be aware of when using culture solutions?

The culture solution should be removed from the refrigerator and left at room temperature for a period of time before experimentation, to avoid the effects of too cold temperatures on the cells.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Great performation

The cells performed well in subculture and are worth recommending to others.

28 Sep 2021

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For research use only. Not for any other purpose.