E11(Mouse)

Cat.No.: CSC-C8794H

Species: Mus musculus, mouse

Source: Kidney

Morphology: Monolayer, adherent

Culture Properties: Monolayer, adherent

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Cat.No.

CSC-C8794H

Description

The E11 cell line has been cloned from the outgrowth of glomeruli which were isolated from H-2kb-tsA58 transgenic mice. The mice carry a temperature-sensitive variant of the SV40 large T antigen under control of the IFN-g-inducible H-2kb promoter. Cells proliferate at 33°C, and they differentiate at 37°C. At present, the cells have been cultured successfully for more than 40 passages without noting phenotypic changes.The species was confirmed by Real-Time PCR. This cell line can express WT1, Lmx1b, nephrin, NEPHI, FAT, P-cadherin, CD2AP, ZO-I, podocalyxin, podoplanin, synpo, podocin, TRPC6 and GAPDH.

Species

Mus musculus, mouse

Source

Kidney

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

Monolayer, adherent

Morphology

Monolayer, adherent

Quality Control

Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^7 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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