DM-3

Cat.No.: CSC-C2541

Species: Human

Source: sarcomatoid mesothelioma

Morphology: adherent, sarcomatoid cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C2541

Description

Established from a pleural biopsy of a 62-year-old man with malignant mesothelioma

Species

Human

Source

sarcomatoid mesothelioma

Recommended Medium

80% DMEM or NCTC-109 + 20% h.i.FBS

Culture Properties

monolayer

Morphology

adherent, sarcomatoid cells growing in monolayers

Karyotype

Human near-diploid karyotype with 16% polyploidy; 46(44-48)<2n>XY; no consistent abnormality detected; resembles published karyotype.

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the disadvantages of cell suspension culture?

The productivity of suspension cultures decreases over extended subculture periods; cells may get damaged by shear conditions.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Shortended experimental timelines

The tumor cell products provided by Creative Bioarray have significantly shortened our experimental timelines.

16 Jan 2023

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For research use only. Not for any other purpose.