D-36

Cat.No.: CSC-C1076

Species: Mouse

Source: mast cells

Morphology: growing in suspension in small clumps

Culture Properties: suspension

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Cat.No.

CSC-C1076

Description

Established from the bone marrow of a DBA/2 mouse in 1986; cells can be used for an IL-10 assay

Species

Mouse

Source

mast cells

Recommended Medium

90% SuperCult® IMDM + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1 ng/ml mouse IL-4 + 1 ng/ml mouse IL-3

Culture Properties

suspension

Morphology

growing in suspension in small clumps

Karyotype

Murine near-diploid karyotype with 2% tetraploidy - 40(37-41)<2n> - centric fusion only rarely present

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.