CI-1

Cat.No.: CSC-C0575

Species: Human

Source: B cell lymphoma

Morphology: clustered cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0575

Description

Established from the ascites of a 38-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL, lymphoblastic, non-convoluted cell type) at diagnosis in 1981

Species

Human

Source

B cell lymphoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

clustered cells in suspension

Karyotype

Human pseudodiploid karyotype with 6% polyploidy - 46(44-49)<2n>XX, t(2;8)(p11;q24), t(14;22)(q32;q11) - matches published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -
Viruses: PCR: EBV +, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5-10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the advantages of immunomagnetic separation?

The main advantages of the immunomagnetic separation technique are the ease of application, flexibility, the relatively low instrumentation needs, and costs related to FACS.

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