CEM-CM3

Cat.No.: CSC-C9357L

Species: Human

Source: T cell; T lymphoblast

Morphology: lymphoblast

Culture Properties: suspension

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Cat.No.

CSC-C9357L

Description

Species: human - female, 4 years old, Caucasian
Histopathology: leukemia, acute lymphoblastic
Note: this line was derived from CCRF-CEM by selecting for resistance to 8-azaguanine; the line has been used as a fusion partner in the production of human-human T cell hybridomas

Species

Human

Source

T cell; T lymphoblast

Recommended Medium

90% RPMI-1640 with 15 mcg/ml 8-azaguanine + 10% h.i. FBS

Culture Properties

suspension

Morphology

lymphoblast

STR DNA Profile

D3S1358: 15
vWA: 18,19
FGA: 23,24
Amelogenin: X
TH01: 6,7
TPOX: 8
CSF1P0: 11
D5S818: 12,13
D13S317: 12
D7S820: 10,13

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule
Shipping Condition: Room Temperature

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the common methods of cell lysis?

In order to obtain as many intracellular metabolites as possible, the cells should be made to be fragmented to the greatest extent possible. Commonly used methods of fragmentation include repeated freeze-thawing, ultrasonic fragmentation, and mechanical homogenization.

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