CAT-13.1E10

Cat.No.: CSC-C6156X

Species: Mouse

Source: hybridoma (CD19)

Morphology: round cells growing as monolayers, some grow in suspension

Culture Properties: suspension

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Cat.No.

CSC-C6156X

Description

Described as secreting a mouse monoclonal antibody (IgG1) CD19; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with EBV-transformed B cells from a patient with MHC class-II deficiency re-expressing class-II antigens

Species

Mouse

Source

hybridoma (CD19)

Recommended Medium

80% DMEM + 20% h.i.FBS + HFCS ("Hybridoma Fusion and Cloning Supplement"for the first 1-2 weeks post-thawing)

Culture Properties

suspension

Morphology

round cells growing as monolayers, some grow in suspension

Quality Control

Mycoplasma: contamination was eliminated with sparfloxacin, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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